Part:BBa_K4613013:Design

T3-M-CPA

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 995
  Illegal BamHI site found at 940
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1105
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 8
  

Design Notes

We fused M-CPA into T3 to immobilize the enzyme and increase the stability and sustainable production of M-CPA. Codon optimization of M-CPA for enhanced Escherichia coli expression.

Source

NAU-CHINA 2023